Share this page on LinkedIn
Share This Page on Google+
Share This Page on Twitter
tell someone about this page print this page
You are here: Contents > 2012 > Volume 21 Number 3 May 2012 > MISCELLANEOUS > Immunohistochemical Detection of uPA, PAI-1, and α-SMA in Aneurysms of Patients with Perimembranous Ventricular Septal Defect

Immunohistochemical Detection of uPA, PAI-1, and α-SMA in Aneurysms of Patients with Perimembranous Ventricular Septal Defect

Juan Qian, Kun Sun, Ping Shen, Min-zhi Yin

Pediatric Intensive Care Unit, and Departments of Pediatric Cardiology and Pediatric Pathology, Shanghai Children’s Medical Center, Shanghai Jiaotong University, Shanghai, P. R. China

Background and aim of the study: A perimembranous ventricular septal defect (PMVSD) may be partially or completely occluded by aneurysms that originate from the tricuspid valve leaflets, though the exact mechanisms of closure remain unknown. It is hypothesized that valvar interstitial cells (VICs) mediate extracellular matrix (ECM) remodeling in aneurysms via the secretion of a serine proteinase and its inhibitor.
Methods: The functional characteristics of VICs in 15 aneurysms and in four normal tricuspid valve leaflets obtained at autopsy were evaluated by detecting the expression of urokinase plasminogen activator (uPA), plasminogen activator inhibitor-1 (PAI-1), and α-smooth muscle actin (α-SMA) in the specimens, using immunohistochemical methods.
Results: uPA and α-SMA were recognized predominantly in VICs located mainly in regions

adjacent to the endothelium and smooth muscle cells of blood vessels. PAI-1 was identified in VICs found mainly in granulation tissues, and in endothelial cells. Two types of granulation tissue (myxoid and fibrous tissue) were associated with aneurysms. Nine aneurysms expressed a high uPA activity and a low PAI-1 activity (uPA/PAI-1 ratio 1.78), while six aneurysms expressed a low uPA activity and a high PAI-1 activity (uPA/PAI-1 ratio 0.14).
Conclusion: The expression of uPA, PAI-1 and
α-SMA in VICs suggests that interactions among these molecules contribute to ECM remodeling during aneurysm formation and development. This provides a potential mechanism for defect closure in patients with PMVSD.


The Journal of Heart Valve Disease 2012;21:377-383

Immunohistochemical Detection of uPA, PAI-1, and α-SMA in Aneurysms of Patients with Perimembranous Ventricular Septal Defect

Click the above hyperlink to view the article, right click (Ctrl click on a Mac) to open in a new browser window or tab.

Purchase this Article

Please click the button below to purchase this article. Single article purchases are provided at $50.00 per article. Upon clicking the button below, single article user account subscription details are requested and, upon successful payment, a single article user account is created. Single articles are availble in your account for seven days after purchase.