Share this page on LinkedIn
Share This Page on Google+
Share This Page on Twitter
tell someone about this page print this page
You are here: Contents > 2013 > Volume 22 Number 5 September 2013 > AORTIC VALVE DISEASE > Side-Specific Characterization of Aortic Valve Endothelial Cell Adhesion Molecules Under Cyclic Strain

Side-Specific Characterization of Aortic Valve Endothelial Cell Adhesion Molecules Under Cyclic Strain

Chelsea T. McIntosh, James N. Warnock

Department of Agricultural and Biological Engineering, Mississippi State University, Mississippi, USA

Background and aim of the study: Aortic valve ectopic calcification occurs exclusively on the fibrosa surface. This may be due to the distinct mechanical environments on either side of the valve, or to the existence of unique, side-specific endothelial subphenotypes. The study aim was to determine if sidespecific endothelial cells (ECs) would differentially express cell-cell and cell-matrix adhesion molecules in response to elevated levels of equibiaxial tensile strain.

Methods: Side-specific porcine aortic valve ECs were isolated and strained at 10% or 20% using a Flexcell 4000T for 24 h, and compared to static controls. The quantity and pattern of distribution of adhesion proteins was then assessed using ELISA and fluorescence microscopy, respectively. The adhesion proteins of interest were platelet endothelial cell adhesion molecule-1 (PECAM-1), β1-integrin, VEcadherin, and vinculin.


Results: Overall, ventricular ECs were more reactive to changes in cyclic strain, with significant increases in VE-cadherin and vinculin at 20% strain. However, the expression of β1-integrin was significantly increased at 20% strain in fibrosa ECs. Expression of PECAM-1 was not significantly changed at all strain levels for both sub-populations of ECs.

Conclusion: Endothelial cells isolated from the fibrosa and ventricularis surfaces of porcine aortic valves showed significantly different expression profiles of cell-cell and cell-extracellular matrix adhesion molecules under elevated tensile strain. These differences in response to cyclic strain suggest that different endothelial sub-phenotypes exist on the fibrosa and ventricularis surfaces of the aortic valve.

The Journal of Heart Valve Disease 2013;22:631-639

Side-Specific Characterization of Aortic Valve Endothelial Cell Adhesion Molecules Under Cyclic Strain

Click the above hyperlink to view the article, right click (Ctrl click on a Mac) to open in a new browser window or tab.

Purchase this Article

Please click the button below to purchase this article. Single article purchases are provided at $50.00 per article. Upon clicking the button below, single article user account subscription details are requested and, upon successful payment, a single article user account is created. Single articles are availble in your account for seven days after purchase.