Growing interest in aortic root replacement and new developments in aortic valve conservation demand a deeper understanding of the normal aortic root anatomy. Ten cryopreserved human aortic roots were pressurized, fixed and measured directly (leaflet free edge and attachment) and using three-dimensional computed tomography imaging software (sinus of Valsalva height and volume). The mean of the measurements of all four parameters yielded a pattern in which the non-coronary sinus (N) structures had the greatest dimensions followed by the right (R) and then the left (L). Non-parametric ANOVA on each of these parameters also showed significant differences among the sinuses yielding a pattern of N>R>L. This pattern determined an angle of tilt between the plane at the base (annulus) and the plane intersecting the sinotubular junction with a mean value of 11[deg]. Linear regression indicated that this angle did not depend on the size of the base (annulus). The possible hemodynamic relevance and surgical implications of these findings need to be explored.