The study goal was to determine whether visualization of single-leg separation (SLS) in cineangiographic images of Björk-Shiley convexo-concave heart valves is correlated to the heart valve dynamics. Images of ten SLS valves were reviewed by three experts, who identified the frames of data in which a SLS was observed. Frames were then correlated to the position of the occluder disk, frame rate of the imaging sequence, and length of cardiac cycle. Reader agreement was also evaluated. The probability of detecting a SLS was not correlated to a point in the cardiac cycle, the length of a cycle, or the image acquisition frame rate. The degree of reader agreement was slight to moderate. In conclusion, visualization of SLS is limited to a small number of image frames and is not correlated to the valve dynamics. In addition, there is a significant amount of reader variability in the interpretation of these images.