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You are here: Contents > 2010 > Volume 19 Number 6 November 2010 > MOLECULAR BIOLOGY > Heart Valve Collagens: Cross-Species Comparison Using Immunohistological Methods

Heart Valve Collagens: Cross-Species Comparison Using Immunohistological Methods

Jacinta F. White, Jerome A. Werkmeister, Stephen L. Hilbert, John A. M. Ramshaw 

CSIRO Molecular and Health Technologies, Bayview Avenue, Clayton, Victoria, Australia, Office of Science and Engineering Laboratories, Center for Devices and Radiological Health, Food and Drug Administration, Rockville, MD, USA

Background and aim of the study: Tissue engineering is an emerging strategy for the development of replacement heart valves where the properties of native tissues are to be replicated. The complexity of the distribution of various collagens in the aortic, mitral, and pulmonary valve leaflets of porcine, bovine, and ovine origin, has been examined.
Methods: Immunohistological and transmission electron microscopy analyses using monoclonal antibodies to types I, III, IV, V and VI collagens were Results: The results indicated that each collagen type

has its own distinct distribution, with minimal variation between heart valve anatomic sites and species. Of particular interest was type VI collagen, which had an asymmetric distribution that was principally localized along the outflow surface of the valve.
Conclusion: Successful tissue engineering constructs of heart valves may need to replicate the complex distribution of different collagens found in heart valve tissues.

The Journal of Heart Valve Disease 2010;19:766-771

Heart Valve Collagens: Cross-Species Comparison Using Immunohistological Methods

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