Share this page on LinkedIn
Share This Page on Google+
Share This Page on Twitter
tell someone about this page print this page
You are here: Contents > 2013 > Volume 22 Number 5 September 2013 > AORTIC VALVE DISEASE > Myeloperoxidase and Progression of Aortic Valve Stenosis in Patients Undergoing Hemodialysis

Myeloperoxidase and Progression of Aortic Valve Stenosis in Patients Undergoing Hemodialysis

Satoko Wada1, Kenichi Sugioka2, Takahiko Naruko3, Yasuyuki Kato4, Toshihiko Shibata4, Takeshi Inoue5, Mayumi Inaba1, Masahiko Ohsawa1, Minoru Yoshiyama2, Makiko Ueda1

Departments of 1Pathology and 2Osaka City University Graduate School of Medicine, Osaka, Departments of 3Cardiology, 4Cardiovascular Surgery and 5Pathology, Osaka City General Hospital, Osaka, Japan

Background and aim of the study: Currently, there is an increased incidence of aortic valve stenosis (AS) in patients undergoing hemodialysis (HD), though the exact mechanisms are not fully understood. Myeloperoxidase (MPO) is a leukocyte-derived enzyme that catalyzes the formation of reactive oxygen species and is an index of oxidative stress. The study aim was to examine, immunohistochemically, the expression of MPO, using surgically resected aortic valve specimens from AS patients undergoing HD.

Methods: The study population consisted of 15 HD patients and 19 non-HD patients with severe AS undergoing aortic valve replacement. Frozen aortic valve samples obtained surgically from AS patients were stained immunohistochemically with antibodies against smooth muscle cells, neutrophils, macrophages, T lymphocytes, CD31, MPO and 4-hydroxy-2-nonenal (4-HNE).


Results: Quantitative analyses showed that the macrophage-positive area, and numbers of T lymphocytes, neutrophils, CD31-positive microvessels and MPO-positive cells in HD patients were significantly higher than in non-HD patients (macrophages, p <0.0001; T lymphocytes, p <0.0001; neutrophils, p <0.0001; CD31, p <0.0001; MPO, p <0.0001). Moreover, the number of MPO-positive cells was positively correlated with CD31-positive microvessels and the 4-HNE-positive macrophage score (CD31, R = 0.73, p <0.0001; 4-HNE, R = 0.49; p <0.005).

Conclusion: These findings suggest that MPO is highly expressed in the aortic valves of AS patients undergoing HD. Furthermore, MPO is positively associated with neovascularization and oxidative stress, which contribute to a rapid progression of AS in HD patients.

The Journal of Heart Valve Disease 2013;22:640-647

Myeloperoxidase and Progression of Aortic Valve Stenosis in Patients Undergoing Hemodialysis

Click the above hyperlink to view the article, right click (Ctrl click on a Mac) to open in a new browser window or tab.

Purchase this Article

Please click the button below to purchase this article. Single article purchases are provided at $50.00 per article. Upon clicking the button below, single article user account subscription details are requested and, upon successful payment, a single article user account is created. Single articles are availble in your account for seven days after purchase.