Contents
Download PDF
pdf Download XML
198901 Views
10782 Downloads
Share this article
Review Article | Volume 10 Issue 1 (, 2004) | Pages 125 - 128
Structural modification and cryopreservation of porcine heart valves for xenotransplantation with reduced immunity
 ,
 ,
 ,
 ,
 ,
 ,
1
Department of Cardiothoracic Surgery, Rabin Medical Center, Petah-Tikva, Israel.
Under a Creative Commons license
PMID : -11206759
Published
Jan. 9, 2001
Abstract

Background and aim of the study: In order to provide valved xenografts with reduced immunity, yet durability comparable with that of homografts, a method for endothelial cell removal was developed.

Methods: Adult porcine valved pulmonary conduits were isolated, washed and incubated in trypsin-EDTA solution. The endothelial cells were flushed free with a stream of culture medium, and the xenografts cryopreserved. Grafts were thawed after three months, and evaluated structurally.

Results: Macroscopic inspection of the grafts revealed no cracks or other morphological damage. Light microscopy revealed mildly edematous changes, and the elastic layers appeared to be preserved. Incubation with trypsin-EDTA solution consistently removed the entire endothelial layer, without obvious damage to the underlying tissues.

Conclusion: With care and appropriate timing, the xenograft endothelium can be selectively removed, leaving the underlying tissue intact. This process may allow further structural manipulations to improve the durability of these grafts.

 

 

 

 

How to cite: Erez, E., Sandbank, J., Stamler, A., Raanani, E., Sharoni, E., Vidne, B. A., & Barak, J. (2001). Structural modification and cryopreservation of porcine heart valves for xenotransplantation with reduced immunity. The Journal of heart valve disease10(1), 125–128.

 
Keywords
Recommended Articles
Research Article
Cross Sectional Study on Nutritional Status and Prevalence of Anemia in Rural Adolescents
Published: 26/06/2013
Download PDF
Read Article
Research Article
To Study the Serum Homocysteine Profile Pattern in AMI & Ischaemic Stroke in Young Adults.
...
Published: 30/08/2025
Download PDF
Read Article
Research Article
Incidence, Clinical Profile, Complications and Outcome of Rodenticide Poisoning in Tertiary Care Hospital
...
Published: 26/08/2025
Download PDF
Read Article
Research Article
To Find Out of the Effects of Mother Nutrition Status on Birth Weight, Anthropometric Measurements of Full-Term Newborn
Published: 07/11/2022
Download PDF
Read Article
© Copyright Journal of Heart Valve Disease