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Review Article | Volume 3 Issue 1 (, 1997) | Pages 105 - 110
Chemical assay of glutaraldehyde incorporation into pericardial tissue
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Biological & Medical Research Department, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
Under a Creative Commons license
PMID : -8162206
Published
Jan. 19, 1994
Abstract

This paper describes the development of a chemical assay to determine the incorporation of glutaraldehyde into pericardial tissue. The procedure is based upon the hydrolysis of the Schiff's bases formed between the glutaraldehyde and the E-amino groups of lysine, the liberated glutaraldehyde being determined as its 2,4-dinitrophenyl-hydrazone by high performance liquid chromatography (HPLC). The HPLC system uses a reverse phase column eluted with a gradient of aqueous acetonitrile (from 60%-70% over 30 minutes). The glutaraldehyde 2,4-dinitrophenylhydrazone is detected at 356 nm. The results show that the assay has sufficient sensitivity (minimum detectable limit 20 pmol of glutaraldehyde 2,4-dinitrophenyl-hydrazone) and reproducibility (+/- 9% at 100 pmol +/- 4% at 1 nmol) to estimate glutaraldehyde incorporation into pericardial tissue. A series of subsequent experiments have documented that temperature and buffer pH are the main determinants affecting glutaraldehyde incorporation into pericardial tissue. Immersion of pericardial tissue in glutaraldehyde solution of pH 9 and 37 degrees C yields an approximately six times greater incorporation than can be achieved if the reaction is performed at 0 degrees C. Further study is underway to relate the amount of Schiff's base bound to that of total incorporated glutaraldehyde.

 

 

 

How to cite: Hughes, H., Lilburn, S., Tipton, S., Aboul-Enein, H. Y., & Duran, C. M. (1994). Chemical assay of glutaraldehyde incorporation into pericardial tissue. The Journal of heart valve disease3(1), 105–110.

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