Mehmet Bulent Vatan1,5, Aysel Kalaycı Yigin2, Ramazan Akdemir1, Mustafa Tarik Agac1, Mehmet Akif Cakar1, Murat Aksoy1, Ersan Tatli1, Harun Kilic1, Huseyin Gunduz1, Derya Guzel3, Keziban Karacan41Department of Cardiology, Sakarya University School of Medicine, Sakarya, Turkey
2Department of Medical Biology and Genetics, Sakarya University School of Medicine, Sakarya, Turkey
3Department of Physiology, Sakarya University School of Medicine, Sakarya, Turkey
4Department of Anatomy, Sakarya University School of Medicine, Sakarya, Turkey
5Electronic correspondence: firstname.lastname@example.org
Background and aim of the study: Mitral chordae tendineae rupture (MCTR) is a progressive disorder which leads to severe mitral regurgitation. Despite its importance, the precise pathogenetic mechanism of MCTR remains unclear. The study aim was to investigate the expression profile of circulating microRNAs (miRNAs) as being potentially involved in the development of MCTR.
Methods: Twenty-one patients with ‘primary’ MCTR, and 30 age- and gender-matched controls, were enrolled in the study. Comparisons were made between the expression levels of circulating miRNAs in MCTR patients and controls. Four target gene databases were used to predict target genes and pathways of differentially expressed miRNAs.
Results: Compared to controls, the expression of 22 miRNAs (hsa-miR-106b-5p, hsa-miR-126-3p, hsa-miR-150-5p, hsa-miR-17-5p, hsa-miR-195-5p, hsa-miR-19a-3p, hsa-miR-19b-3p, hsa-miR-20a-5p,
hsa-miR-21-5p, hsa-miR-222-3p, hsa-miR-223-3p, hsa-miR-23a-3p, hsa-miR-25-3p, hsa-miR-92a-3p, hsa-miR-93-5p, hsa-miR-26b-5p, hsa-miR-30e-5p, hsa-miR-373-3p, hsa-miR-15b-5p, hsa-miR-16-5p, hsa-miR-191-5p, hsa-miR-26a-5p) were significantly down-regulated in the MCTR group. Bioinformatic analysis indicated that the following potential miRNA targets and pathways are commonly related to the development of MCTR: MMPs, TIMP-2,TGFBR2, VEGFA, PIK3R2, NRAS, PPP3CA, PPP3R1, PTGS 2 were predicted as putative targets of 13 of these miRNAs.
Conclusion: The present study is the first to describe altered miRNA expression in patients with MCTR. Bioinformatic analysis has revealed that target genes involved in MCTR development were regulated by miRNAs.
The Journal of Heart Valve Disease 2016;25:580-588
|Altered Plasma MicroRNA Expression in Patients with Mitral Chordae Tendineae Rupture|
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